A200 - Amino Acid Analyzer
The newest amino acid analyzer on the market, featuring a narrow bore
column for reduced reagent consumption.
The A200 Amino Acid Analyzer is a dedicated,
compact, and complete analysis system, specialized for the routine
analysis of amino acids by postcolumn derivatization with ninhydrin. The
integrated unit consists of specially developed components to provide the
user with the most optimized system on the market.
The elution buffer system consists of six nontoxic ready-prepared
buffers, compatible for both hydrolysis and physiological analyses. These
buffers are easily accessible through their arrangement in an eluent
carousel in the upper compartment. Rather than refilling buffer bottles
and risking contamination, the buffer bottles can be simply replaced when
necessary. A liquid level alarm ensures that sufficient buffer is always
available during an analysis.
The eluent carousel holds all six eluent buffers (buffers A through F)
as well as the ninhydrin reagent and the reactor wash solution. The
eluents are electronically selected by six single solenoid valves which
can also support gradient elution. The dual piston pump draws in the
eluent and reagent simultaneously through its ceramic double pump head.
Thereby ensuring an optimal and constant mixing proportion of buffer to
reagent in the reactor.
Base line disturbances are minimized by the inclusion of a precolumn
which effectively removes interfering ammonia. Once through the separation
column, the separated amino acids are mixed with the ninhydrin reagent
inside the high temperature reactor to yield colored complexes. They are
then detected in the flow photometer at 570 nm and at 440 nm in a second
photometer. The column temperature is accurately controlled by a
high-quality Peltier heating element to provide for the highest possible
The automated sample injection system can maintain up to 120 samples in
a cooled environment so that sensitive samples can be used over an
extended period of time. The injection volume can be adjusted in 1 µl
increments from 1 to 40 µl.
The narrow bore stainless steel column and precolumn have a service
life of approximately 1,000 samples, dependent on the sample matrix. With
these columns, a complete separation of the 43 amino acids of the
physiological standard can be separated in less than 95 minutes. Depending
on the analysis, a temperature gradient can be programmed for the
separation column within the range of 20°C and 100°C. Complete computer
control and data analysis are
performed using ChromGate® Software.
New control and analysis software make setup and running very user
Graphical Representation of a Method:
Loading the Autosampler
Here is some summary information in the form of a 2 page brochure in
Amino Acid Analyzer Summary
Here is some applications information in the form of a 9 page brochure in
Amino Acid Analyzer Application
For more information please contact us directly.