Why is online degassing so important?
Online degassing is important when doing HPLC, FPLC, GPC, and uHPLC because degassing will remove dissolved gas therefore avoiding bubble formation. Degassing will improve quantitative analysis. Online degassing replaces helium sparging.
Outgassing is a term used to describe dissolved gases coming out of solution. This occurs in HPLC systems where rough surfaces produce nucleation sites for bubble formation. Bubbles entering the HPLC systems will present a false signal to the detector.
Outgassing and bubble formation create problems for HPLC, FPLC, GPC and uHPLC systems.
Low pressure mixing: systems are more prone to bubble formation. The solvents used for the mobile phase are mixed before the pump. Outgassing affects the volume of liquid after the valve and before the pump because the valves are proportioned based on time and cannot discern whether there are bubbles in the liquid or not. This results in an inaccurate gradient.
- With high pressure mixing each solvent has its own pump and mixing takes place after the pumps. This configuration is less prone to outgassing issues.
- Outgassing creates bubbles in the flow cell
- Outgassing disrupts the column bed in FPLC ruining the separation process
- Bubble formation can be the cause of many performance issues, both with low pressure and high-pressure mixing
Our online degasser modules use proprietary technology. A flat membrane is incorporated on both sides of the cassette to provide maximum surface area with minimal volume. We optimize the efficiency of the membrane by precisely controlling its thickness to maximize degassing efficiency. Vacuum degassing is optimized because the membrane has a high gas permeability, ensuring both efficient and continuous gas removal, regardless of the flow rates. This technology also enhances the lifetime of the degasser. This innovative technique enables the highest degassing efficiency in the industry. Our breakthrough in technology hugely improves traditional Teflon tube degassers that are commonplace today.
We offer 2-8 channel modules that are available to handle analytical to semi-prep applications. All modules work with HPLC, UHPLC, FPLC, and GPC. Custom OEM configurations are available.
Outgassing occurs when gasses that have dissolved into the solvent or buffer cause bubbles in pumps and detectors. Therefore, good chromatographic separations require degassing the solvents or buffers.
While the solvent flows through the Teflon AF® membrane, the dissolved gases are removed from the solvent by means of a vacuum on the other side of the membrane. Typically 50% of dissolved gas must be removed from the solvent stream to avoid bubble formation. Online degassers are used at universities, research institutions, and laboratories in the following areas: biochemistry analysis, chemical analysis, pharmaceutical analysis, food and beverage analysis, environmental analysis, chiral analysis and protein purifications. Our degassers are engineered for superior performance and the highest degassing efficiency in the industry. We use Teflon AF® in all of our analytical and semi-prep degassers. The benefit of online degassing is that it is a continuous process as opposed to helium sparging which is typically done prior to the chromatographic run. After the solvents are helium sparged, gases are reintroduced into the solvent providing inconsistent degassing which degrades over time.
Read MoreWhy is it important to eliminate bubbles through online degassing?
- Bubbles in the flow cell cause erroneous spikes in your chromatograms
- Bubbles in solvents cause inaccurate and irreproducible gradient information
- Bubbles in solvent steam create inaccurate flow rates causing changes in chromatographic retention times
- Bubbles cause noisy baselines
- When Dissolved gas forms bubbles they affect the accuracy, precision and performance of your chromatography system.
If gas dissolves in liquid it is likely to cause disturbances in fluidic systems. Gas molecules can form bubbles when pressure or temperature changes. Using our degassers optimizes the performance of HPLC, uHPLC and FPLC systems.
Degassers are the solution to air bubbles in the fluidic lines which cause problems and lead to production disruptions or delays.
Degassers improve reliability because they remove dissolved gases from fluids prior to outgassing. This prevents bubbles from forming and causing problems.
Benefits of using online degassing:
- improves baseline stability
- efficiently removes dissolved gases from fluid stream
- prevents bubbles from forming
- reduces noise
- reduces start-up times
- ensures more constant and accurate results
Degassers for HPLC systems: Analytical degassers are degassers used for this degassing solvent in HPLC and uHPLC systems.
Our analytical degassers include:
AZE 03-1- 4 channel degasser biocompatible
AZE 02-AZURA DG 2.1S- 2 channel degasser
A 5335- analytical 2 channel degasser for GPC
Semi-prep degassers are designed to handle flow rates up to 30 mls/min
Our Knauer semi-prep/degasser is an analytical 2 channel degasser for GPC. The model number is Smartline A 5328.
Preparative degasser- preparative degassers are used for higher flow pumping systems or chromatography systems that can handle flow rates up to 1000 mls/min.
Preparative degassers options are:
AZE 03 – Preparative 4 channel degasser
AZE 02-1 Preparative 2 channel degasser
ICON Scientific, Inc
